Glucose metabolism and glutamate analog acutely alkalinize pH of insulin secretory vesicles of pancreatic -cells
نویسندگان
چکیده
Kazuhiro Eto,1,3 Tokuyuki Yamashita,1 Kenzo Hirose,2 Yoshiharu Tsubamoto,1 Edward K. Ainscow,5 Guy A. Rutter,5 Satoshi Kimura,1 Mitsuhiko Noda,3,4 Masamitsu Iino,2 and Takashi Kadowaki1,3 Departments of 1Metabolic Diseases and 2Pharmacology, Graduate School of Medicine, University of Tokyo, Tokyo 113-8655; 3CREST of Japan Science and Technology Corporation, Saitama 332-0012; 4Institute for Diabetes Care and Research, Asahi Life Foundation, Tokyo 100-0005, Japan; and 5Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, United Kingdom
منابع مشابه
Glucose metabolism and glutamate analog acutely alkalinize pH of insulin secretory vesicles of pancreatic beta-cells.
We studied acute changes of secretory vesicle pH in pancreatic beta-cells with a fluorescent pH indicator, lysosensor green DND-189. Fluorescence was decreased by 0.66 +/- 0.10% at 149 +/- 16 s with 22.2 mM glucose stimulation, indicating that vesicular pH was alkalinized by approximately 0.016 unit. Glucose-responsive pH increase was observed when cytosolic Ca2+ influx was blocked but disappea...
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